View Poll Results: have you had candida and cured it?

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  • yes I have had it and no longer suffer from candida

    8 61.54%
  • no, I have never had it

    2 15.38%
  • no, I have it and still suffer

    3 23.08%
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Thread: Has anyone here had Candida and cured it?

  1. Link to Post #41
    New Zealand Unsubscribed
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    Default Re: Has anyone here had Candida and cured it?

    Hi there,

    I do not know if this product is available in your country, but it has been proven to be remarkable in the treatment of Candida (amongst many other ailments).

    This is the New Zealand website, but the product is based in & originates from the USA.

    https://www.cellfood.co.nz/

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    United States Avalon Member RunningDeer's Avatar
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    Default Re: Has anyone here had Candida and cured it?

    Quote Posted by KiwiElf (here)
    Hi there,

    I do not know if this product is available in your country, but it has been proven to be remarkable in the treatment of Candida (amongst many other ailments).

    This is the New Zealand website, but the product is based in & originates from the USA.

    https://www.cellfood.co.nz/
    Thanks for the info, KiwiElf. It’s available elsewhere, too. CELLFOOD

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  4. Link to Post #43
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    Default Re: Has anyone here had Candida and cured it?

    I have a friend who runs the "Simply Well Migraine Protocall" her name is Marya Gendron. https://www.facebook.com/groups/Simp...group_activity

    Marya suffered for years with candida and to my knowledge she has cured herself. She is a brilliant woman and has also cured herself from migraines. I would get in touch with her. She is well versed and studied and would have a great deal to add that could be shared.

    From the Heart,
    Kristin

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  6. Link to Post #44
    Canada Avalon Member Daughter of Time's Avatar
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    Default Re: Has anyone here had Candida and cured it?

    I don't have time to read the posts above so I don't know if this has been mentioned:

    Hydrogen Peroxide - food grade - I think it is 30% so it's very strong.

    Start with 1 drop in a full glass of water, taken on an empty stomach, preferably first thing in the morning.

    Day 1 - 1 drop
    Day 2 - 2 drops
    Day 3 - drops, etc., until you reach the maximum

    The safe maximum is 1 drop per 10 pounds of body weight so if you weigh 100 lbs, then your maximum should be 10 drops. Stay on your maximum, according to your weight, for 2 weeks and then reverse until you go back to 1 drop per day and keep taking it daily for maintenance.

    If you start to feel nauseous then do not increase until the nausea stops, once the nausea stops, increase another drop. I hope this is clear.

    Turpentine seems to work for many people. I tried it and it had very adverse effects on my digestive system but I could handle Hydrogen peroxide as described above with no problems. I felt very energetic once I'd done the cleanse. It kills fungi and bacteria and other pathogens but it does not kill good bacteria.

    Eat fermented foods. Take probiotics. Eat fruit but only the low glycemic kind, like plums and apples. Avoid oranges, grapes and bananas as they contain a lot of sugar. Avoid all refined flours and processed foods. Avoid grains until the condition has cleared. Only old grains like millet, amaranth, buckwheat and brown rice are allowed, but even these should be consumed sparingly.

    While vegan diets work for many, they do not work for me. If being vegan works for you, that is great! But you must avoid sugars wheat, potatoes, and anything which converts into sugar. You can do some research about which foods convert into sugar. All vegetables are good except potatoes.

    Hydrogen peroxide is very potent stuff so make sure you don't get it anywhere near your eyes as it can burn them. If you get it on your hands, the affected part will turn white, but this goes away quickly. Make sure children do not go near it. Keep it in the freezer. Available at almost any health food store.

    Good luck to you in finding the right solution. It's out there. I'm sure there is a lot of great advice on this thread beside the one in my post.

    With love,

    Daughter of Time
    Last edited by Daughter of Time; 23rd March 2019 at 17:03.

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    United States Avalon Member Savannah's Avatar
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    Default Re: Has anyone here had Candida and cured it?

    When I stopped eating sugar (grains, corn, corn syrup, maple syrup, et) the candida stopped in about 3 months. The fungus eats sugar thus you are starving it out. I didn't change my diet for that reason but noticed it was one oaf the many benefits of eating healthy. Also when I had chronic fatigue syndrome I had severe candida. Most people get it when they have a cold or flu (coated tongue) because our immune system is on overdrive or weakened. Thus you may have another issue that is primary and candid is a reaction.

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  10. Link to Post #46
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    Default Re: Has anyone here had Candida and cured it?

    I posted here a few days ago, and my posts keep disappearing. Help!

    Among worms, parasites, virus, bacteria and fungus, fungus is the most tenacious and hard to get rid of.
    Candida is fungus and its resembles (some) cancers in its behavior.
    Like cancer cells, everyone has candida to a certain degree, and the question is to keep it in check.

    There are herb combinations and homeopathy to deal with candida. If it's on the skin, you can use good quality essential oils, but the fungus comes from inside the body, and not just on the surface.
    The symptoms include athlete's foot, jock itch, lines in the nails, deformed nails (toes and fingers), emotional swings, etc., and white people seem to be more prone than Asians.

    That's why you have to be careful when you buy Chinese medicine from a Chinese pharmacy because the product almost always contains mushrooms.

    By the way, if you see a few worms come out, don't think you have dewormed successfully.
    Just like roaches, if you kill one, there are still 99 hiding in some places. Worms are the same way. Deworm regularly and never eat raw fish, among other things.

  11. Link to Post #47
    Scotland Moderator Billy's Avatar
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    Default Re: Has anyone here had Candida and cured it?

    Quote Posted by Didgevillage (here)
    I posted here a few days ago, and my posts keep disappearing. Help!

    Among worms, parasites, virus, bacteria and fungus, fungus is the most tenacious and hard to get rid of.
    Candida is fungus and its resembles (some) cancers in its behavior.
    Like cancer cells, everyone has candida to a certain degree, and the question is to keep it in check.

    There are herb combinations and homeopathy to deal with candida. If it's on the skin, you can use good quality essential oils, but the fungus comes from inside the body, and not just on the surface.
    The symptoms include athlete's foot, jock itch, lines in the nails, deformed nails (toes and fingers), emotional swings, etc., and white people seem to be more prone than Asians.

    That's why you have to be careful when you buy Chinese medicine from a Chinese pharmacy because the product almost always contains mushrooms.

    By the way, if you see a few worms come out, don't think you have dewormed successfully.
    Just like roaches, if you kill one, there are still 99 hiding in some places. Worms are the same way. Deworm regularly and never eat raw fish, among other things.
    Hi Didge.
    I can see two previous posts from you in this thread, both on page 2, posts 39 and 40.
    One here.
    http://projectavalon.net/forum4/show...=1#post1282248

    And here.
    http://projectavalon.net/forum4/show...=1#post1282269

    Hope this helps.
    When you express from a fearful heart in the now moment, You create a fearful future.
    When you express from a loving heart in the now moment, You create a loving future.

    Have no fear, Be aware and live your lives journey from a compassionate caring nurturing heart to manifest a compassionate caring nurturing future. Billyji


    Peace

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  13. Link to Post #48
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    Default Re: Has anyone here had Candida and cured it?

    Billy, thank you. I'm not good at these things.
    I will keep my posts as they are, because they contain somewhat different information.
    Additionally, Hulda Clark zapper also helps.

    Be it sugar or tea/coffee, it is difficult to keep its consumption down to zero.
    In conjunction with another post about water fasting, we have to deal with:
    fungus
    viruses
    bacterial
    parasites
    worms
    and chemicals in the body.

    Worms and fungi are very compatible with each other and they seem to thrive in a polluted environment (in a human), so that you need to deworm if you want to get rid of fungus, vice versa.

    Viruses hide in a bacterium, and bacteria hide in a parasite or worm, so it's more efficient to kill bigger guys first.

  14. Link to Post #49
    Avalon Member xylo's Avatar
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    Default Re: Has anyone here had Candida and cured it?

    I hardcore eliminated carbs and used anti fungal herbs, which did leave me experiencing die-off symptoms. I used the recommended probiotics which also resulted in dieoff symptoms. Finally I switched to a product called SF-77 which is a form of castor bean oil. That oil purportedly converts yeast back from fungal form so the immune system can deal with it. No die off symptoms and I think it works. I’m feeling much better now.
    Last edited by xylo; 24th March 2019 at 23:41. Reason: Spelling

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    Default Re: Has anyone here had Candida and cured it?

    Killing viruses via frequencies
    I don't know if anything ever came of this research but the possibilities seem endless, if it hasn't or doesn't become suppressed which it likely has, as with anything revolutionary and extremely workable, it seems....
    Although students of Rife probably don't need any further proofs!

    New Way to Kill Viruses: Shake Them to Death
    By Michael Schirber | February 5, 2008
    https://www.livescience.com/7472-kil...ake-death.html
    "Scientists may one day be able to destroy viruses in the same way that opera singers presumably shatter wine glasses. New research mathematically determined the frequencies at which simple viruses could be shaken to death.

    "The capsid of a virus is something like the shell of a turtle," said physicist Otto Sankey of Arizona State University. "If the shell can be compromised [by mechanical vibrations], the virus can be inactivated."

    Recent experimental evidence has shown that laser pulses tuned to the right frequency can kill certain viruses. However, locating these so-called resonant frequencies is a bit of trial and error.

    "Experiments must just try a wide variety of conditions and hope that conditions are found that can lead to success," Sankey told LiveScience.

    To expedite this search, Sankey and his student Eric Dykeman have developed a way to calculate the vibrational motion of every atom in a virus shell. From this, they can determine the lowest resonant frequencies.

    As an example of their technique, the team modeled the satellite tobacco necrosis virus and found this small virus resonates strongly around 60 Gigahertz (where one Gigahertz is a billion cycles per second), as reported in the Jan. 14 issue of Physical Review Letters.

    A virus' death knell

    All objects have resonant frequencies at which they naturally oscillate. Pluck a guitar string and it will vibrate at a resonant frequency.

    But resonating can get out of control. A famous example is the Tacoma Narrows Bridge, which warped and finally collapsed in 1940 due to a wind that rocked the bridge back and forth at one of its resonant frequencies.

    Viruses are susceptible to the same kind of mechanical excitation. An experimental group led by K. T. Tsen from Arizona State University have recently shown that pulses of laser light can induce destructive vibrations in virus shells.

    "The idea is that the time that the pulse is on is about a quarter of a period of a vibration," Sankey said. "Like pushing a child on a swing from rest, one impulsive push gets the virus shaking."

    It is difficult to calculate what sort of push will kill a virus, since there can be millions of atoms in its shell structure. A direct computation of each atom's movements would take several hundred thousand Gigabytes of computer memory, Sankey explained.

    He and Dykeman have found a method to calculate the resonant frequencies with much less memory.

    In practice

    The team plans to use their technique to study other, more complicated viruses. However, it is still a long way from using this to neutralize the viruses in infected people.

    One challenge is that laser light cannot penetrate the skin very deeply. But Sankey imagines that a patient might be hooked up to a dialysis-like machine that cycles blood through a tube where it can be hit with a laser. Or perhaps, ultrasound can be used instead of lasers.

    These treatments would presumably be safer for patients than many antiviral drugs that can have terrible side-effects. Normal cells should not be affected by the virus-killing lasers or sound waves because they have resonant frequencies much lower than those of viruses, Sankey said.

    Moreover, it is unlikely that viruses will develop resistance to mechanical shaking, as they do to drugs.

    "This is such a new field, and there are so few experiments, that the science has not yet had sufficient time to prove itself," Sankey said. "We remain hopeful but remain skeptical at the same time." "
    ************************************************** **************

    Efficient Structure Resonance Energy Transfer from Microwaves to Confined Acoustic Vibrations in Viruses
    December 2015
    https://www.nature.com/articles/srep18030
    "Efficient Structure Resonance Energy Transfer from Microwaves to Confined Acoustic Vibrations in Viruses
    Szu-Chi Yang, Huan-Chun Lin, Tzu-Ming Liu, Jen-Tang Lu, Wan-Ting Hung, Yu-Ru Huang, Yi-Chun Tsai, Chuan-Liang Kao, Shih-Yuan Chen & Chi-Kuang Sun
    Scientific Reports volume 5, Article number: 18030 (2015) | Download Citation

    Abstract
    Virus is known to resonate in the confined-acoustic dipolar mode with microwave of the same frequency. However this effect was not considered in previous virus-microwave interaction studies and microwave-based virus epidemic prevention. Here we show that this structure-resonant energy transfer effect from microwaves to virus can be efficient enough so that airborne virus was inactivated with reasonable microwave power density safe for the open public. We demonstrate this effect by measuring the residual viral infectivity of influenza A virus after illuminating microwaves with different frequencies and powers. We also established a theoretical model to estimate the microwaves power threshold for virus inactivation and good agreement with experiments was obtained. Such structure-resonant energy transfer induced inactivation is mainly through physically fracturing the virus structure, which was confirmed by real-time reverse transcription polymerase chain reaction. These results provide a pathway toward establishing a new epidemic prevention strategy in open public for airborne virus.

    Introduction
    In the past few decades, tremendous efforts have been made to kill airborne viruses such as severe acute respiratory syndrome (SARS) coronavirus or influenza viruses, which have caused catastrophic illness worldwide. Current airborne virus epidemic prevention to be used in public space includes strong chemical inactivation, UV irradiation, and microwave thermal heating. All these methods affect the open public. In 1980s, Robach et al.1 and Cerf 2 demonstrated that ultrasonic energy can be absorbed by viruses. In 2000, Babincová et al.3 hypothesized that viruses can be inactivated by generating the corresponding resonance ultrasound vibrations of viruses, which is in the GHz region. Based on this hypothesis, several groups started investigating the vibrational modes of viruses in this frequency range4,5,6,7. Recently we demonstrated that dipolar mode of the confined acoustic vibrations (CAVs) inside viruses can be resonantly excited by microwaves of the same frequency with a resonant microwave absorption effect8. The observed microwave resonance absorption phenomenon indicates a possible structure-resonant energy transfer (SRET) effect from electromagnetic waves (EM waves) to CAVs of viruses. Theoretically this SRET process is an efficient way to excite the vibrational mode of the whole virus structure due to a 100% energy conversion of a photon into a phonon of the same frequency, but the overall SRET efficiency is also related to the mechanical properties of the surrounding environment9, which influences the quality factor of the oscillator (virus). A study on the SRET efficiency to inactive virus is thus highly desired and it will determine if this SRET phenomenon provides a solution to inactivate airborne viruses in open public for epidemic prevention.

    In this article, we show that SRET from microwave to virus can be efficient enough so that airborne virus was inactivated with reasonable microwave power density safe for the open public. To investigate the SRET efficiency from EM waves to CAVs in viruses, we first developed a theoretical model to describe the relation between the induced stress and the field magnitude of the illuminating microwave. Since the viruses could be inactivated when the induced stress fractures the structure of viruses, we propose to explore the SRET efficiency from microwaves to viruses through measuring the virus inactivation threshold. Based on the proposed model, we studied the inactivation ratio of influenza A (H3N2) virus at dipolar-mode-resonance and off-resonance microwave frequencies as well as with different microwave powers. Plaque assay10 was then applied to calculate the titer of virus samples before and after the microwave illumination. Our results indicate efficient SRET from microwave to viruses, which resulted in higher inactivation ratio of viruses at the dipolar resonant frequency. At the resonant frequency, the microwave power density threshold for H3N2 inactivation was found to be below the IEEE safety standard, also agreeing well with our developed theoretical model. The real-time reverse transcription polymerase chain reaction (real-time RT-PCR) method11 was further performed to confirm that the main inactivation mechanism is through physically fracturing the viruses while the RNA genome was not degraded by the microwave illumination, supporting the fact that our studied SRET mechanism is fundamentally different from the microwave thermal heating effect. These results provide a pathway toward establishing a new epidemic prevention strategy in open public for airborne virus.

    Modelling
    From the transmission electron microscope images, people knew that the virions of influenza viruses are basically spherical balls packing genomes inside. Since the protein and genome have similar mechanical properties8, for the estimation of dipolar vibration frequencies, we treat the virion as a homogenous sphere.

    Dipolar Mode of a Homogeneous Sphere
    Due to the spatial confinement, not only electronic but also acoustic energy quantization has been observed in low dimensional systems such as quantum dots and nano-wires. In 1882, Lamb studied the torsional (TOR) and spheroidal (SPH) modes of a homogeneous free sphere by considering the stress-free boundary condition on the surface12. Among these modes, the SPH mode with allows dipolar coupling13 and the corresponding eigenvalue equation can be expressed as14,15:


    where , is the spherical Bessel function of the first kind, ω is the angular frequency of the vibrational mode, R is the radius of the nano-sphere, cland ctare longitudinal and transverse sound velocities respectively. A comparison between the commonly observed breathing mode and the dipolar mode can be found in Supplementary online. the Since the dipolar mode of a nano-sphere cannot be detected by the light scattering experiments16, it was not observed until a previous study of the resonant excitation of dipolar mode through THz wave or microwave excitations17,18 when the core and shell of the nano-sphere have permanent charge separation. Once the resonantly oscillating electric field was applied to the nano-sphere, opposite displacement between core and shell was generated, thus excited the dipolar mode vibrations. Compared with the breathing () and quadrapolar () modes, dipolar mode () is the only SPH mode to directly interact with the EM waves whose wavelength is much longer than the particle’s size. Due to the permanent charge separation nature of viruses, in 2009, dipolar coupling with CAVs is confirmed to be the mechanisms responsible for microwave resonant absorption in viruses by treating spherical viruses as free homogeneous nanoparticles8,9.

    Figure 1 shows the simulated displacement field of the dipolar mode (calculated by the finite element method, COMSOL Multiphysics, COMSOL, Inc.) of a homogeneous sphere (mass density and viscoelastic properties are constant throughout the sphere). We define the relative displacement direction of the dipolar mode as the z-direction, which will also be the field direction of the applied EM waves discussed in the next section. By plotting the displacement field of the x-z plane (y = 0) of the sphere, the opposite displacement between the core and shell regions can be clearly observed in Fig. 1(b). Meanwhile Fig. 1 (c) shows the side view of the distortion of the x-y plane of the sphere at different z locations, which concludes that the maximum distortion occurs on the equatorial plane (z = 0) of the sphere. Figure 1(d) shows the top view of the displacement field of the equatorial plane (z = 0). It is interesting to find out that the magnitude of averaged positive displacement (inner region) is 1.27 times the magnitude of the averaged negative displacement (outer region), while positive and negative displacements occupy 42% and 58% area, respectively. Furthermore one can find that the maximum magnitude of the displacement, occurring either at the very center or the outer surface of the equatorial plane, is approximately twice of the averaged magnitude of the displacement.

    A Damped Mass-Spring Model
    In this work, microwaves were applied to excite the dipolar resonance of the whole virus structure. By exciting the dipolar mode of the nanosphere, core and shell with opposite charge distributions would move in opposite directions and will resonate like a damped mass-spring system17. Our following analysis is similar to the Drude-Lorentz model describing the light-atom interaction, which connects a damped mass-spring system to the quantum-mechanical electronic resonant transitions. In the damped mass-spring system by adopting the reduced mass (m*) of core and shell in the analysis, the relative motion of the displacement can be shown in the following equation:


    where z is the relative displacement between the core and shell; b is the damping coefficient, which is related to the surrounding environment; k is the effective spring constant of this system. By assuming z(t) proportional to exp(iωt), one can solve the complex angular frequency of the resonator as:


    Therefore the decay rate of the oscillation equals to the imaginary part of the frequency (b/2m*), which corresponds to ω0/2Q19:


    The intrinsic resonance angular frequency (ω0) of this system is (k/)0.5. Q is the quality factor of the resonator. From equation (4), stronger damping increased the energy transfer between the resonator and its surrounding environment, which decreases the confinement of the vibration and leads the lower Q. Now we approximate that a spherical virus is like a homogeneous sphere but with opposite and equal charges in the core and shell regions. When the oscillating electric field (cos) of microwaves is applied to the system, forced displacements would be induced with the same frequency as the applied microwaves. The equation of motion now needs to include the force induced by the applied electric field (qE), where q is the total amount of charge distributed in the core and shell region of a virus:


    We describe the forced displacement as , where A is the amplitude of the forced displacement and is the phase delay between the displacement and the applied electric field. By solving the particular solution of this differential equation, one can obtain the phase delay and the amplitude of the forced oscillating displacement as


    and


    The instantaneous power absorption of this system is then described as the following equation, where v is the velocity of the oscillating motion17:


    By integrating over one full cycle, one can obtain the average power absorption from the system:


    Then the absorption cross-section of the virus can be obtained by setting the input power flux as with20


    where is the relative permittivity in the system and c is the speed of light in vacuum.

    Threshold to Fracture a Virus
    With oscillating dipolar vibration to inactivate a virus, the most possible mechanism is to fracture the most outer surface of the equatorial plane (z = 0) due to the location of the maximum distortions, as illustrated in Fig. 1 (c,d). For influenza viruses, this corresponds to the lipid membrane of the envelope. To estimate the maximum induced stress on the equatorial plane, we divide the maximum induced force by the area of the shell region (defined by the moving direction in the approximate model) on the equatorial plane. Following above discussion, we found that the maximum induced stress is twice the average value and the shell region covers 58% of the equatorial plane:


    If the required stress threshold to fracture a virus can be obtained, the threshold electric field magnitude of the incident microwaves can also be obtained by using:


    Figure 2 shows the threshold magnitude of the incident electric field at different frequencies with different Q based on equation (12) with a fixed threshold value. One can observe that the minimum of the threshold electric field magnitude occurs when the applied frequency is closed to the intrinsic resonant frequency. Moreover cavity quality factor Q plays a major role. By changing the pH value of the solution, charge status of viral surface can be modulated, which affects the Q of the vibration. For example, previous studies indicated that the cavity Q of spherical viruses ranges between 2–10 by raising the pH value of the solution from 5.4 to 7.48. With increased Q, more energy can be confined inside the resonator, which leads to much lower microwave field threshold magnitude at the resonant frequency.

    To experimentally study the efficiency of the SRET from microwaves to CAVs of spherical viruses, influenza A virus subtype H3N2 was used. H3N2 is a subtype of influenza A virus that causes flu. Such viruses can infect birds and mammals and are increasingly abundant in seasonal influenza, which kills an estimated 6309 people in the United States each year, including pneumonia and influenza causes21. Based on previous studies, the averaged mass and the diameter of the H3N2 virus are 161 MDa22 and 100 nm23. Here we approximate the structure of the virus as a nanosphere with a core-shell structure of opposite charge distribution. The shell (90% of the total mass) contains lipid, neuraminidase (NA), hemagglutinin (HA), and M-protein. The core (10% of the total mass) includes RNA and RNP. The reduced mass (m*) of virus is thus 14.5 MDa. From the literature24, force with 400 pN applied on the AFM tip can fracture the lipid envelope. Since the radius of the tip was 30 nm24, the threshold stress to fracture the shell was 0.141 MPa (). In order to calculate the threshold magnitude of the electric field to fracture H3N2 virus following equation (12), some important parameters such as q, Q and ω0 of the studied H3N2 virus has to be obtained by measuring the microwave absorption spectrum of viruses.

    As shown in Fig. 3(a) we covered the structure of the coplanar waveguide (CPW) by the microfluidic channel with a 1.25 mm-long sensing zone (L) in order to measure the microwave absorption spectrum of viruses. This microwave microfluidic channel can provide a microwave bandwidth over 40 GHz. The measured results were summarized in Fig. 3(b). As the figure shows, the power absorption ratio (α) by the virus at the resonant frequency (8.2 GHz) was 21% and the Q was only 1.95 by measuring the full width at half maximum of the spectrum. Since the density of viruses (N) in the solution was 7.51014 m−3, experimental absorption cross section of the virus at the resonant frequency can be calculated by the equation below:


    From equation (10), the theoretical absorption cross-section of the virus at the resonant frequency is:


    By setting of the PBS at 8.2 GHz as 67.1325,  = can be obtained by comparing equation (13) and equation (14).

    So far, all parameters for estimating electric field threshold in equation (12) are obtained. By substituting threshold Pstress = 0.141 MPa,  = , m* = 14.5 MDa, Q = 1.95 and ω0 = 2π × 8.22 GHz into equation (12), threshold magnitude of electric field to fracture virus at different frequencies of microwave can be calculated. The result is shown in Fig. 3(c). In order to compare with the following inactivation experiments, our estimated threshold magnitude of electric field at 6, 8 and 10 GHz are 103.3, 86.9 and 137.1 V/m, respectively. The minimum threshold occurs close to 8 GHz due to resonance, and sufficient internal stress to fracture virus can be expected to be more efficiently generated by weaker electric field.

    Based on the IEEE Microwave Safety Standard, the spatial averaged value of the power density in air in open public space shall not exceed the equivalent power density of 100(f/3)1/5 W/m2 at frequencies between 3 and 96 GHz (f is in GHz)26. This corresponds to 115 W/m2 at 6 GHz, 122 W/m2 at 8 GHz, and 127 W/m2 at 10 GHz for averaged values of the power densities in air. Assuming all the microwave power in air 100% transmitted into a specimen, and by taking the dielectric constant of water 71.92 (6 GHz), 67.4 (8 GHz), and 63.04 (10 GHz)25 for calculation, this safety standard then corresponds to the average electric field magnitude of 101 V/m (6 GHz), 106 V/m (8 GHz), 110 V/m (10 GHz) inside the water-based specimens. It is interesting to notice that the required threshold electric field magnitudes at the resonant frequency (86.9 V/m) to fracture H3N2 viruses as shown in Fig. 3(c) are within the IEEE Microwave Safety Standard (106 V/m), indicating high SRET efficiency, even though the quality factor of the H3N2 virus is low.

    Results
    Virus Inactivation Experiments – Frequency dependency
    To investigate the resonant effect, we first measured the residual viral infectivity of influenza A virus after illuminating microwave of different frequencies. As shown in Fig. 4(a), viral samples were placed below the horn antenna. The sponge under the sample was used to decrease the reflection of the microwave. To check the inactivation ratio, illuminated viruses were then analyzed by plaque assay to measure the residual infectivity of viruses. We compared the titer of illuminated viruses (Ntest) and the titer of unilluminated control sets (Ncontrol) to calculate the inactivation ratio (1 − Ntest/Ncontrol) at different frequencies between 6–12 GHz.

    Based on Fig. 3(c), the field intensity threshold for inactivating H3N2 virus ranges between 86.9–236.3 V/m, which corresponds to 82.3–564 W/m2, for microwaves between 6 and 12 GHz. Since the aperture size of our horn antenna was 9.8 cm × 7.1 cm. The required threshold power input ranges from 0.57 W to 3.92 W for 6–12 GHz microwaves. We thus first applied 6.3 W (38 dBm) fixed microwave power, which is higher than all the threshold power input, into the horn antenna for the frequency dependency studies. After considering the transmission coefficient of our horn antenna, this experimental condition corresponded to 765 – 882 W/m2 average illuminated power density on the sample surface, corresponding to the field intensity inside the specimen of 260–296 V/m respectively. For 8–8.4 GHz microwave at the resonant frequency, the average illuminated power density was about 810 W/m2, equivalent to 273 V/m effective field intensity inside the sample. We thus expect to observe the inactivation effect throughout the studied spectral range. As been summarized in Fig. 4(b), a frequency dependent inactivation ratio can be observed in our experiments, with a peak located at the resonant frequency of the dipolar mode while higher than 50% inactivation ratio can be observed throughout the studied frequency range. At 8.4 GHz, the measured titer count was zero, indicating 100% inactivation ratio, which means that the remaining active viral concentration was smaller than the system sensitivity of 10 pfu/mL. This result indicates at least a three-order of magnitudes attenuation on the virus titer, when the microwave frequency was tuned to the dipolar mode resonant frequency with the electric field intensity 3 times higher than the threshold. The illuminated average power density was roughly 6.7 times higher than the IEEE safety standard for the 8–8.4 GHz cases. It is important to notice that the power density is proportional to the square of the field intensity.

    Virus Inactivation Experiments – Power density dependency
    To further investigate the efficiency of this SRET effect from microwave to virus and the threshold effect, we further measured the inactivation ratio of H3N2 virus with different power densities at the resonant frequency ~8 GHz of the confined acoustic dipolar mode. Our theoretical model predicted an inactivation threshold field intensity of 86.9 V/m, corresponding to an average microwave power density of 82.3 W/m2 in specimen. Since we assume all power can transmit from air to specimen, power density in air is also 82.3 W/m2, which is 1.48 times lower than the IEEE safety standard. Figure 5 (b) summarized the measured inactivation ratio for 4 different average microwave power densities of 820, 320, 82, and 51 W/m2 in air, corresponding to an effective field intensity inside samples of 274, 171, 87, and 68 V/m, respectively. It is noted that the experiment with 82 W/m2 in air was performed in a different experimental setup, as shown in Fig. 5(a). A significant threshold effect can be observed when the effective field intensity inside samples started to be on the order of or exceed the estimated threshold. A 38% inactivation ratio can be observed with a field intensity of 87 V/m, while the inactivation ratio dropped drastically to only negligible 6% with a slightly lower 68 V/m field intensity. With a 3 times higher field intensity than the threshold, the inactivation ratio saturated at a 100% value.

    Discussions
    Compared with the simple theoretical model for threshold estimation as summarized in Fig. 3(c), our result agrees qualitatively and surprisingly quantitatively. First, in our experiments, we observed a strong resonant effect on the virus inactivation ratio at the dipolar oscillation frequency of 8.4 GHz, thus indicating that the observed virus inactivation after microwave illumination was due to the proposed SRET from microwave to virus through dipolar coupling. Second, at the resonant frequency, we do observe H3N2 virus inactivation by illuminating 82 W/m2 (lower than the IEEE safety standard in public space) 8 GHz microwaves on our viral solution, corresponding to an average 87 V/m electric field intensity inside the solution, confirming that our proposed simple model to estimate the field threshold (86.9 V/m) to structurally fracture the virus is quantitatively correct, especially combining the observed threshold effect as discussed above. With a low resonator quality factor (around and less than 2 for H3N2), we also observed virus inactivation in off-resonant frequencies (6-12GHz), following a trend predicted with our model. However for off-resonant frequencies, the simple harmonic oscillator model seems to always estimate a lower threshold in the Stoke-side (lower-frequency-side) of the resonant frequency than the anti-Stoke-side (high-frequency-side). For example, at DC (0 frequency) it still predicts a relatively low threshold field magnitude to fracture the virus. This is different from our observation. We observe that the anti-Stoke-side is with a better inactivation ratio than the Stoke-side, and the source for disagreement should be the over-simplification of the adopted model.

    Our result regarding the efficient SRET to inactivate virus with a low microwave power has a profound meaning. As we introduced, in the past few decades, tremendous efforts have been made to kill airborne viruses such as SARS or influenza A, which have caused catastrophic illness worldwide. Active airborne viruses are always transported inside tiny water droplets, thus similar to our experimental condition. A strategy for airborne virus epidemic prevention in open public is thus highly desired. Our finding represents the first possible mechanism to inactivate airborne viruses without affecting the open public, since the required microwave power could be within the IEEE safety standard. Comparing this work with traditional microwave thermal inactivation, previous works27,28 used a microwaves oven with more than 100 W to heat the phage suspension. The inactivation ratio could reach almost 100% by increasing the temperature of the phage suspension to 80 °C. In our case, 100% inactivation were achieved with 6.3 W (38 dBm) as the input power into the horn antenna at 8.4 GHz. A power reduction by more than a factor of 15 is achieved. It is however not possible to directly compare the irradiating power density, since the irradiating area was not provided in previous literatures. Nevertheless our work still shows sharp contrast to current methodologies, including strong chemical inactivation, UV irradiation, and microwave thermal heating with 100 W microwave power27,28, which are not safe for the open public.

    A previous study29 has shown that to inactivate human H3N2 viruses through thermal heating, the temperature need to be higher than 55 °C. Compared with the 82 W/m2 radiated microwave power density (0.63 W required power input) in our resonant inactivation case, the current microwave thermal heating method to inactivate virus usually requires more than 100 W microwave power at 2.45 GHz27,28, which is way beyond the safety standard, in order to raise the sample temperature to be higher than 60 °C for protein denature. It is known that the microwave thermal heating has a weak frequency dependency between 6–12 GHz, and this is not the case for our frequency dependent result as shown in Fig. 4(b). To confirm that our observation is not due to the microwave thermal heating effect, we had monitored the sample temperature change during the microwave illumination experiments with a radiated power density of 486 W/m2 at a frequency of 6 GHz by using an infrared thermal imaging camera with a temperature accuracy of 0.05 °C (CHCT, P384-20). The temperature rise after 15 minutes radiation was 7 °C, from 27.5 °C up to 34.5 °C. We thus exclude the possible contribution of microwave thermal heating effect to inactivation H3N2 viruses under our experimental condition.

    To double-confirm our proposed mechanism that the inactivation was through physically fracturing the structure of viruses, we established a fractured virus model by freezing the virus samples with liquid nitrogen and thawed immediately and repeated several times. We then preformed real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction) experiment for RNA amplification in order to compare the results after microwave illumination with the established fractured-virus model. Without protein denature, the established fractured-virus model allowed the viral RNA content to be extracted after fracturing. We then performed the RT-PCR experiments to amplify the extracted viral RNA after fracturing either through the frozen fracturing model or after microwave resonant irradiation. Figure 6(a) summarized our results. The applied average microwave power density was 320 W/m2, the microwave frequency was tuned to the dipolar mode resonant frequency of 8.35 GHz, and the illumination time was 15 minutes. To avoid possible existing viral RNA in solution before the microwave illumination, we pretreated the virus samples by adding RNase to degrade the existing RNA outside the viral particles. As can be revealed in Fig. 6(a), the control fractured-virus model (shown as “control”) showed the same RNA amplification trend with excellent quantitative agreement with the RNase-pretreated samples after microwave resonant illumination (shown as “pretreat”). Obvious increase of copies in PCR can be observed after cycle 15. We have also performed two post-treat groups to double confirm the effect of the RNase. For post-treat groups, RNase was added right after the microwave resonant illumination. Even if the virus particles were fractured, the released RNA would be degraded by RNase, we did not expect to detect the viral RNA. As been also confirmed by our RT-PCR experiment as shown in Fig. 6(a), we were not able to detect the signal of RNA for the post-treat groups even after 45 cycles.

    Since all the RNAs outside the virus surface in the solution were eliminated before the microwave illumination, for the pretreat samples the only way to detect the RNA signal after the illumination was to fracture the virus so that the RNA can be released. This result was also in good agreement on the amount of amplified RNA in the control cases. These facts confirmed that the inactivation of viruses by illuminating microwaves at the dipolar mode resonant frequency was through physically fracturing the lipid-envelope of the influenza virus without denaturing the viral RNA.

    To show that the investigated SRET effect can be applied to deactivate viruses other than H3N2, we have also performed the real-time RT-PCR experiment on H1N1 virus. The result was shown in Fig. 6(b). The applied microwave frequency was 7 GHz, while the applied average microwave power density was 308 W/m2, corresponding to an effective electric field of 167 V/m inside the specimens. The rest of the experimental conditions were the same as that of Fig. 6(a). Similar results can be observed, indicating that the same SRET induced inactivation effect can also occurs in virus other than H3N2.

    In summary, we investigated the structure resonance energy transfer from microwave to CAVs of H3N2 virus in water-based solution. The efficiency of such energy transfer was investigated through exploring the virus inactivation ratio. Based on the proposed damped mass-spring model and the experimentally measured microwave absorption cross-section of a single virus, threshold magnitude of electric field to fracture viruses at different illuminated frequencies can be estimated. After the illumination by the microwave, the plaque assay experiment indicated that the inactivation ratio reaches its maximum at the resonant frequency of the dipolar resonance. The real-time RT-PCR experiment double confirmed that the main inactivation mechanism was through physically fracturing viruses without degrading viral RNA genome. This work not only theoretically and experimentally demonstrates a new energy transfer mechanism between EM waves and viruses, but also indicates an efficient SRET effect. Our results have important implications for the interaction between microwaves and biological tissues, which is a highly concerned public issue. With an observed inactivation threshold with a microwave power density within the IEEE safety standard, the demonstrated SRET mechanism also provides a pathway toward establishing a new epidemic prevention strategy in open public for airborne viruses.

    Methods
    H3N2/H1N1 Sample Preparation
    To prepare the H3N2 and H1N1 virus samples, Madin-Darby canine kidney (MDCK) cells were confluent grown in 75 cm2 flasks, inoculated by influenza A virus H3N2 (H090103, NTU Hospital) or H1N1 (NTUH135/2009, NTU Hospital) with multiplicity of infection (MOI) of 0.01 and incubated at 37 °C in a 5% CO2 incubator for 2–3 days. When the cytopathic effect (CPE) of the inoculated cells reached to 3 + (75%), we harvested the viral supernatant. The viruses were aliquot and stored at −80 °C for further use.

    Microwave Absorption Spectrum Measurement
    The microwave absorption spectrum measurement was performed by combining the coplanar waveguide (CPW) circuit with a microfluidic channel. As shown in Fig. 3(a), we covered the structure of the CPW by the microfluidic channel with a 1.25 mm-long sensing zone (L). The gap between the signal electrode and the ground electrode of the CPW was 25 μm. In order to decrease microwave loss on the electrodes, the gold layer thickness of the electrode was 1.2 μm. On the surface of electrodes, we grew a thermal isolator layer of silicon dioxide on the sensing zone by Plasma Enhanced Chemical Vapor Deposition (PECVD) to lower the temperature rise of fluids due to microwave dielectric heating30. We then used a network analyzer (Anritsu, MS2028C) as the source to measure the absorption spectrum of H3N2 viruses from 6 GHz to 14 GHz. The virus particle density in the solution was 7.5 × 1014 m−3. The spectrum of the solution without viruses was first measured as a reference; solution with viruses was later measured. By removing the solution background, the microwave absorption spectrum of H3N2 viruses in solution was thus obtained (Fig. 3(b)) following a procedure similar to ref. 8.

    Microwave Illumination Measurement
    In our experiments, we used two different microwave sources, depending on the utilized microwave power: a network analyzer (Anritsu, MS2028C) or an Yttrium iron garnet (YIG) oscillator. Then the microwave signal was amplified by a power amplifier (QPJ-06183640) and radiated from the horn antenna (ELECTRO-METRICS, EM-6969). The aperture size of the antenna was 9.8 cm × 7.1 cm. To avoid damage on oscillator and amplifier caused by back reflection, we added an isolator and a directional coupler. All the microwave systems were put in a P2 class flow hood. The antenna was directed toward the bottom (Fig. 4(a)) or the side (Fig. 5(a)) of flow hood and the microwave was normally incident on either microscope slides (Fig. 4(a)) or acrylic cuvettes (Fig. 5(a)) at a distance of 5 cm below the exit of horn antenna. To avoid large reflection from the metal hood surface, the microscope slide or cuvette was put in a plastic dish supported by a broad band pyramidal absorber. For each measurement, the viral solution (7.5 × 1014 m−3 particle density) under illumination was drop on the slide and covered with a cover glass or was contained inside the cuvettes. Under such an experimental geometry, we illuminated viruses for 15 minutes at various microwave frequencies or at various microwave powers. After illumination, we used buffer to wash-down and collect the viruses, which introduced a 10-fold dilution of virus concentration. Then the illuminated viral solutions, together with the control sets were sent for plaque assay.

    Quantitative Plaque Assay Analysis
    To measure the activity of viruses, we employed a quantitative plaque assay. The MDCK cells used for plaque assay were grown in 6 wells plates by adding 3 mL of cells (2 × 105/mL) to individual well. After confluent growth of MDCK cells in plates, the cells were successively washed with phosphate buffer saline (pH 7.2) and Eagles’ MEM with 2 μg/mL TPCK trypsin. After washed, 100 μL of the ten-fold serial dilution of viruses were added into each well. For better virus adsorption, the inoculated cells were incubated at 37 °C in a 5% CO2 incubator for one hour. After then, the virus inoculums were removed and the Eagles’ minimal essential medium with 2 μg/mL TPCK trypsin and 0.5% agarose was added. After the gel formation, the plates were put in a 5% CO2 incubator for at least 42–48 hrs. After incubation, the plates were fixed with 10% formalin for 1 hour. After pour off agarose, the fixed cells were stained with crystal violet for 15 min and washed with tap water. Below certain virus concentration, the plaques wouldn’t overlap each other seriously and can be counted unambiguously. Considering the corresponding dilution factor, the plaque forming unit per mL (pfu/mL) of the original virus can thus be quantified. The titer measurement will be performed three times on the same sample to reduce quantization error. Quantified by the plaque assay, the concentration of active viruses in our prepared viral solutions was around 107/ml for H1N1 and H3N2.

    Real-time PCR Analysis
    The sample RNA was extracted and amplified by the RT and quantitative real-time PCR (Primerdesign Precision OneStep™ qRT-PCR Mastermix) with primer AMF (sequence: 5′-GAGTCTTCTAACCGAGGTCGAAACGTA-3′), primer flu-AR (sequence: 5′-CAAAGCGTCTACGCTGCAGTCC-3′) and flu A (5′-FAM-tttgtgtttacgctcaccgt-TAMRA-3′) probe. For control group, RNase was added for 10 minutes digestion and stopped with RNase inhibitor. Then viruses were fractured artificially by freeze-thaw treatment. We thus were able to observe that the RNA signal of virus rose after 15 cycles of the amplification. For pre-treated groups, the RNase treatment were done and stopped before 8 GHz microwave illumination in order to make sure that RNA outside the viral envelope was eliminated in the first place. For the post-treat groups, RNase was added right after the illumination. If the virus particles were fractured, the released RNA would be degraded by RNase.

    Additional Information
    How to cite this article: Yang, S.-C. et al. Efficient Structure Resonance Energy Transfer from Microwaves to Confined Acoustic Vibrations in Viruses. Sci. Rep. 5, 18030; doi: 10.1038/srep18030 (2015).

    See the link for References etc.
    https://www.nature.com/articles/srep18030
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    Default Re: Has anyone here had Candida and cured it?

    I've heard the theory that you have to scrape Candida out of your digestive tract by eating lots of high fiber food such as brown rice, while also taking probiotics. Nobody with Candida issues should consume wheat or gluten.

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    Default Re: Has anyone here had Candida and cured it?

    Brown rice was touted as a "cure-all" by the extremist Japanese macrobiotics.
    But in reality, brown rice is loaded with aflatoxin.

    There are articles on certain probiotics doing more damage, and I would just stick to acidophilus.

    Candid cannot be reduced to zero, just like parasites and worms who always manage to sneak into your system through food and air. The issue is to keep them under control, exactly like cancer cells which everyone has.

    Avoid extremes like vegans and enjoy foods and drinks (with moderation).

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    Default Re: Has anyone here had Candida and cured it?

    Quote Posted by Didgevillage (here)
    Brown rice was touted as a "cure-all" by the extremist Japanese macrobiotics.
    But in reality, brown rice is loaded with aflatoxin.

    There are articles on certain probiotics doing more damage, and I would just stick to acidophilus.

    Candid cannot be reduced to zero, just like parasites and worms who always manage to sneak into your system through food and air. The issue is to keep them under control, exactly like cancer cells which everyone has.

    Avoid extremes like vegans and enjoy foods and drinks (with moderation).
    Rice is one of the few foods that nobody is allergic or sensitive to; it's the basis of the elimination diet. There's nothing extreme about eating rice, white or brown. Most of Asia eats it daily. Visit an Asian-American and you'll probably see a 25-pound bag of rice in their kitchen. Don't let it get wet and you won't have to worry about aflatoxin.

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    Default Re: Has anyone here had Candida and cured it?

    Macrobiotics are extreme.
    Brown rice is their Savior.

    Don't let it rain so that rice plants won't get. Is that what you're saying?

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    Canada Avalon Member TomKat's Avatar
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    Default Re: Has anyone here had Candida and cured it?

    Quote Posted by Didgevillage (here)
    Macrobiotics are extreme.
    Brown rice is their Savior.

    Don't let it rain so that rice plants won't get. Is that what you're saying?
    Eating ideologically, are we?
    Rice grows in water, so the rain shouldn't bother it.

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    Default Re: Has anyone here had Candida and cured it?

    https://onlinelibrary.wiley.com/doi/...sfa.2740460308

    "Aflatoxins (AFS) are toxic and carcinogenic fungal metabolites. Aflatoxin B1 is the most toxic and has been classified as a Group I carcinogen by the International Agency for Research on Cancer (IARC). Samples of imported rice were analyzed for their AFS content. Finley ground rice subsamples were extracted with water/methanol (100:150 v/v) followed by purification with Immunoaffinity columns (IAC). AFS purified from extracts were determined with RP-HPLC-FLD using post column electrochemical derivatization with a Kobra Cell. Concentrations of aflatoxin B1 and total AFS in test rice samples were ≤ 0.123 and ≤ 2.58 µg/kg, respectively. Tween 80 improved recoveries (86 and 106%) of aflatoxin B1 and aflatoxin G1 from brown rice. Recoveries of Aflatoxin B2 and aflatoxin G2 were substantially reduced (non-detected to 27%) by Tween 80 used in IAC cleanup of brown rice extracts. Visible dense growth of Aspergillus parasiticus (food isolate) occurred at 25°C but higher aflatoxin B1amounts (23.9-39.3 µg/kg) accumulated when the mold grew at 37°C in rice seeds stored for three week. It could be concluded that levels of aflatoxin B1 and total AFS in rice samples were within the permissible amounts of the EU and other international legislations."
    https://www.researchgate.net/publica...nation_in_Rice

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    Default Re: Has anyone here had Candida and cured it?

    Quote Posted by Flash (here)

    Thanks Bill for the vic vaporub into, i wonder which ingredient in it does the job of treating fungus.
    Camphor

    Eucalyptus oil

    Cedarleaf oil

    Nutmeg oil

    Petrolatum

    Thymol

    Turpentine oil

    Menthol


    https://vicks.com/en-us/safety-and-f...ks-vaporub-faq
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    Default Re: Has anyone here had Candida and cured it?

    I can report that cayenne capsules definitely reduce candida infection in my prostate. I got some 00 gel caps and filled them with cayenne pepper (you can get these little devices on ebay for filling gel caps). I take a couple every morning. Very noticeable difference.

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    Default Re: Has anyone here had Candida and cured it?

    Quote Posted by rebecca7411 (here)
    I looked this up and read that turpentine kills beneficial bacteria as well as harmful bacteria, making it questionable. Someone with Candida needs to build up beneficial bacteria in their bodies
    wrong. It repels them... it doesnt kill them. antibiotics is way worse.

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    Default Re: Has anyone here had Candida and cured it?

    Candida Solutions – The Science Behind the Cure-audio now playing
    NaturalHealth365 Talk Hour

    "Our Guest: Dr. Jeffrey McCombs is a third-generation graduate of Palmer College of Chiropractic. Licensed in California and Illinois, he is a member of the California and Illinois Chiropractic Associations, the International Association for Specialized Kinesiologists, and the American Holistic Health Association. Dr. McCombs has created a detoxification and dietary plan for candida that counters the detrimental effects of antibiotics and re-establishes the normal body flora, detoxification pathways, and regeneration cycles of a vital, youthful, and healthy body. On this program, we’ll focus our attention on the nutritional, environmental, emotional, structural, and biochemical aspects of candida. How do we know if we have candida and why is it important NOT to kill it. Is fruit o.k. or not? What about candida testing – is it a waste of time? And, most importantly, you’ll discover the best ways to rid yourself of candida – using the ‘Dr. McCombs plan.’ Don’t miss it!"
    Go to the link to play the audio--live now
    https://www.naturalhealth365.com/free-show/
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