jackovesk
22nd August 2011, 03:01
Artist uses Spider-Goat silk to make 'bulletproof skin'
August 22, 2011
http://resources3.news.com.au/images/2011/08/22/1226119/452291-jalila-essaidi.jpg
Bio-artist Jalila Essaidi, who used Professor Randy Lewis's special spider-goat silk to create "bulletproof skin". Picture: AP
Scientist spins spider-goat silk fibres
Artist uses fibres for 'bulletproof skin'
"What curious person could say no?"
A BIO-ART project to create bulletproof skin has given a Utah researcher even more hope his genetically-engineered spider silk can be used to help surgeons heal large wounds and create artificial tendons and ligaments.
Researcher Randy Lewis and his collaborators gained worldwide attention recently when they found a commercially viable way to manufacture silk fibres using goats and silkworms that had spider genes inserted into their makeup.
http://resources0.news.com.au/images/2011/08/22/1226119/453504-bulletproof-skin.jpg
A high-speed camera image provided by Essaidi showing a .22 calibre bullet, fired at a reduced speed, hitting but not breaking the skin. Picture: AP
Spider silk is one of the strongest fibres known and five times stronger than steel. Professor Lewis's fibres are not that strong, but are much stronger than silk spun by ordinary worms.
With Prof Lewis's help, Dutch artist Jalila Essaidi conducted an experiment weaving a lattice of human skin cells and silk that was capable of stopping bullets fired at reduced speeds.
"Randy and I were moved by the same drive I think, curiosity about the outcome of the project," Essaidi said.
"Both the artist and scientist are inherently curious beings."
Prof Lewis thought the project was a bit off the wall at first, Essaidi acknowledged.
"But in the end, what curious person can say no to a project like this?" she said.
Essaidi, who used a European genetics-in-art grant to fund her project at the Designers & Artists 4 Genomics Awards, initially wanted to use Prof Lewis's spider silk from goats to capitalise on the "grotesque factor" of the mammal-spider combination.
But Prof Lewis didn't yet have enough of the spider goat silk to send hundreds of yards to Essaidi. So he sent her spools of silk from silkworms he had genetically engineered in a fashion similar to the goats.
Essaidi initially intended to fire .22 calibre bullets at the "skin" stretched in a frame. But she decided to place the "skin" on a special gelatin block used at the Netherlands Forensic Institute.
Using a high-speed camera, she showed a bullet fired at a reduced speed piercing the skin woven with an ordinary worm's silk But when tested with Lewis' genetically engineered worm's silk grafted between the epidermis and dermis, the skin didn't break.
Neither silk was able to repel a bullet fired at normal speed from a .22 calibre rifle.
"We were more than a little surprised that the final skin kept the bullet from going in there," Prof Lewis said of the tests at reduced speed.
"It still ended up 2 inches into the torso, so it would not have saved your life. But without a doubt the most exciting part for us is the fact that they were able to recreate the skin on top of our fibres. It's something we haven't done. Nobody has worked in that area."
Essaidi was intrigued by the concept of spider silk as armour, and wanted to show that safety in its broadest sense is a relative concept, hence bulletproof.
"If human skin would be able to produce this thread, would we be protected from bullets?" "If human skin would be able to produce this thread, would we be protected from bullets?" she wondered on her blog.
"I want to explore the social, political, ethical and cultural issues surrounding safety in a world with access to new biotechnologies."
She said it is legend that Achilles was invulnerable in all of his body except for his heel.
"Will we in the near future due to biotechnology no longer need to descend from a godly bloodline in order to have traits like invulnerability?" she asked.
"If human skin would be able to produce this thread, would we be protected from bullets?"
Prof Lewis downplayed the potential bulletproof applications of his research.
"I certainly would not discount that, but I don't see that as a tremendous application at the moment," he said.
He said bulletproof vests already exist. But being able to grow cells and use the material to replace large amounts of human skin could be significant for surgeons trying to cover large wounds, or treat people with severe burns.
He said the material's strength and elasticity would enable doctors to cover large areas without worrying about it ripping out — a big advantage over small skin grafts.
Prof Lewis couldn't give a time frame for such a use because it would require FDA approval. But he hoped to do some animal testing within two years, and noted spider silk already has proven very compatible with the human body.
The next step is to generate more material to test what cells will grow on it — made easier with the "transgenic" silk worms and milk from goat spiders.
The real stuff is still the holy grail for fibres and textiles but not the easiest to come by as evidenced by an 11-by-4 foot tapestry unveiled two years ago at the New York Museum of Natural History that took millions of spiders to complete.
"We know some skin cells will grow (on our fibres), but can we get cells that make ligaments and tendons grow," Prof Lewis said.
He said it may be easier to use the genetically engineered silk to make materials better than actual ligaments or tendons.
Essaidi, meanwhile, said she has plenty of wild ideas but wants to transplant the bulletproof skin.
She said Geert Verbeke, director of Verbeke Foundation in Belgium, the biggest Eco/BioArt museum, wants to wear the skin "as an ode to BioArt".
Back at Utah State's bio-manufacturing facility in Logan, Utah, Prof Lewis just started breeding for the next round of milking in January. He has about three dozen of the genetically-engineered goats and extracts proteins from the special milk, then spins them in a way that replicates the spider's method, resulting in a strong, light-weight fibre.
"Nothing is as strong as the natural fibre, yet," Prof Lewis said of spider silk.
"But we are working on solving that problem."
http://www.heraldsun.com.au/technology/sci-tech/artist-uses-spider-goat-silk-to-create-bulletproof-skin/story-fn5iztw3-1226119457700
Spider-goats!? Read the original story here...
Meet Spider Goat - the DNA-enhanced web-flinging nanny that may one day knit bones
http://resources1.news.com.au/images/2010/05/17/1225867/619153-spider-goat.jpg
Professor Randy Lewis said no animals were harmed during the making of spider webs from goat's milk / news.com.au
Spider DNA spliced into goats
Milk protein becomes web
ON a farm in Wyoming, USA, goats are being milked for their spider webs.
And if that sounds bizarre, molecular biologist Randy Lewis claims that within two years, spider silk milked from goats could replace your body's tired or strained tendons and ligaments - maybe even bones.
Professor Lewis and his team at the University of Wyoming have successfully implanted the silk-making genes from a golden orb spider into a herd of goats and are now, finally, producing one of nature's strongest products in useable quantities.
The technology is cutting edge, but the science isn't. Spider silk has been used for centuries to dress wounds with varying degrees of success, but the problem has until now been how to get it.
"We needed a way to produce large quantities of the spider silk proteins," Prof Lewis told news.com.au.
"Spiders can't be farmed, so that route is out and since they make six different silks, even that would not work if you could."
Spiders also had a tendency to eat each other, so milking one thread from six out of a solo spider was clearly never going to service the entire human race.
Prof Lewis and his team singled out the "dragline" - the outer strand of the web - as the strongest of the six types of silk.
They spliced the DNA that creates the silk into a female goat's DNA, then waited for it to give birth and start lactating.
"(The splicing) turned out to be relatively easy as there are known gene promoters that only produce expression in the mammary gland during lactation," he said.
"Those were hooked up to our spider silk genes."
After the milk is collected, it's taken back to a laboratory where the silk protein is filtered out. It solidifies when exposed to air and is wound onto a roller.
Prof Lewis said the team collected about four metres of silk for every four drops of protein they gathered.
The pure material had a wide range of medicinal applications as sutures and binding agents - including ligament replacement - but its use could extend well beyond our hospitals.
"If it works, frankly one of the first applications is maybe fishing line," Prof Lewis said.
"I think we will be testing real world applications in less than two years (but) when they reach market is really beyond my control."
And in case you were wondering, no goats were harmed during the making of spider silk milk.
Prof Lewis said there was no evidence to suggest the goats in the experiment behaved any differently to regular goats, in either physiology or "psychology".
One day, the burden could be lifted even from goats.
Prof Lewis said the technology could have farm applications - he told Science Nation they were developing the same technology for alfalfa.
http://www.youtube.com/watch?v=ktgACq4zcAU&playnext=1&list=PL0A80C6A04C15983F
http://www.news.com.au/technology/sci-tech/meet-spider-goat-the-dna-enhanced-web-flinging-nanny-that-may-one-day-knit-your-bones/story-fn5fsgyc-1225867617374
August 22, 2011
http://resources3.news.com.au/images/2011/08/22/1226119/452291-jalila-essaidi.jpg
Bio-artist Jalila Essaidi, who used Professor Randy Lewis's special spider-goat silk to create "bulletproof skin". Picture: AP
Scientist spins spider-goat silk fibres
Artist uses fibres for 'bulletproof skin'
"What curious person could say no?"
A BIO-ART project to create bulletproof skin has given a Utah researcher even more hope his genetically-engineered spider silk can be used to help surgeons heal large wounds and create artificial tendons and ligaments.
Researcher Randy Lewis and his collaborators gained worldwide attention recently when they found a commercially viable way to manufacture silk fibres using goats and silkworms that had spider genes inserted into their makeup.
http://resources0.news.com.au/images/2011/08/22/1226119/453504-bulletproof-skin.jpg
A high-speed camera image provided by Essaidi showing a .22 calibre bullet, fired at a reduced speed, hitting but not breaking the skin. Picture: AP
Spider silk is one of the strongest fibres known and five times stronger than steel. Professor Lewis's fibres are not that strong, but are much stronger than silk spun by ordinary worms.
With Prof Lewis's help, Dutch artist Jalila Essaidi conducted an experiment weaving a lattice of human skin cells and silk that was capable of stopping bullets fired at reduced speeds.
"Randy and I were moved by the same drive I think, curiosity about the outcome of the project," Essaidi said.
"Both the artist and scientist are inherently curious beings."
Prof Lewis thought the project was a bit off the wall at first, Essaidi acknowledged.
"But in the end, what curious person can say no to a project like this?" she said.
Essaidi, who used a European genetics-in-art grant to fund her project at the Designers & Artists 4 Genomics Awards, initially wanted to use Prof Lewis's spider silk from goats to capitalise on the "grotesque factor" of the mammal-spider combination.
But Prof Lewis didn't yet have enough of the spider goat silk to send hundreds of yards to Essaidi. So he sent her spools of silk from silkworms he had genetically engineered in a fashion similar to the goats.
Essaidi initially intended to fire .22 calibre bullets at the "skin" stretched in a frame. But she decided to place the "skin" on a special gelatin block used at the Netherlands Forensic Institute.
Using a high-speed camera, she showed a bullet fired at a reduced speed piercing the skin woven with an ordinary worm's silk But when tested with Lewis' genetically engineered worm's silk grafted between the epidermis and dermis, the skin didn't break.
Neither silk was able to repel a bullet fired at normal speed from a .22 calibre rifle.
"We were more than a little surprised that the final skin kept the bullet from going in there," Prof Lewis said of the tests at reduced speed.
"It still ended up 2 inches into the torso, so it would not have saved your life. But without a doubt the most exciting part for us is the fact that they were able to recreate the skin on top of our fibres. It's something we haven't done. Nobody has worked in that area."
Essaidi was intrigued by the concept of spider silk as armour, and wanted to show that safety in its broadest sense is a relative concept, hence bulletproof.
"If human skin would be able to produce this thread, would we be protected from bullets?" "If human skin would be able to produce this thread, would we be protected from bullets?" she wondered on her blog.
"I want to explore the social, political, ethical and cultural issues surrounding safety in a world with access to new biotechnologies."
She said it is legend that Achilles was invulnerable in all of his body except for his heel.
"Will we in the near future due to biotechnology no longer need to descend from a godly bloodline in order to have traits like invulnerability?" she asked.
"If human skin would be able to produce this thread, would we be protected from bullets?"
Prof Lewis downplayed the potential bulletproof applications of his research.
"I certainly would not discount that, but I don't see that as a tremendous application at the moment," he said.
He said bulletproof vests already exist. But being able to grow cells and use the material to replace large amounts of human skin could be significant for surgeons trying to cover large wounds, or treat people with severe burns.
He said the material's strength and elasticity would enable doctors to cover large areas without worrying about it ripping out — a big advantage over small skin grafts.
Prof Lewis couldn't give a time frame for such a use because it would require FDA approval. But he hoped to do some animal testing within two years, and noted spider silk already has proven very compatible with the human body.
The next step is to generate more material to test what cells will grow on it — made easier with the "transgenic" silk worms and milk from goat spiders.
The real stuff is still the holy grail for fibres and textiles but not the easiest to come by as evidenced by an 11-by-4 foot tapestry unveiled two years ago at the New York Museum of Natural History that took millions of spiders to complete.
"We know some skin cells will grow (on our fibres), but can we get cells that make ligaments and tendons grow," Prof Lewis said.
He said it may be easier to use the genetically engineered silk to make materials better than actual ligaments or tendons.
Essaidi, meanwhile, said she has plenty of wild ideas but wants to transplant the bulletproof skin.
She said Geert Verbeke, director of Verbeke Foundation in Belgium, the biggest Eco/BioArt museum, wants to wear the skin "as an ode to BioArt".
Back at Utah State's bio-manufacturing facility in Logan, Utah, Prof Lewis just started breeding for the next round of milking in January. He has about three dozen of the genetically-engineered goats and extracts proteins from the special milk, then spins them in a way that replicates the spider's method, resulting in a strong, light-weight fibre.
"Nothing is as strong as the natural fibre, yet," Prof Lewis said of spider silk.
"But we are working on solving that problem."
http://www.heraldsun.com.au/technology/sci-tech/artist-uses-spider-goat-silk-to-create-bulletproof-skin/story-fn5iztw3-1226119457700
Spider-goats!? Read the original story here...
Meet Spider Goat - the DNA-enhanced web-flinging nanny that may one day knit bones
http://resources1.news.com.au/images/2010/05/17/1225867/619153-spider-goat.jpg
Professor Randy Lewis said no animals were harmed during the making of spider webs from goat's milk / news.com.au
Spider DNA spliced into goats
Milk protein becomes web
ON a farm in Wyoming, USA, goats are being milked for their spider webs.
And if that sounds bizarre, molecular biologist Randy Lewis claims that within two years, spider silk milked from goats could replace your body's tired or strained tendons and ligaments - maybe even bones.
Professor Lewis and his team at the University of Wyoming have successfully implanted the silk-making genes from a golden orb spider into a herd of goats and are now, finally, producing one of nature's strongest products in useable quantities.
The technology is cutting edge, but the science isn't. Spider silk has been used for centuries to dress wounds with varying degrees of success, but the problem has until now been how to get it.
"We needed a way to produce large quantities of the spider silk proteins," Prof Lewis told news.com.au.
"Spiders can't be farmed, so that route is out and since they make six different silks, even that would not work if you could."
Spiders also had a tendency to eat each other, so milking one thread from six out of a solo spider was clearly never going to service the entire human race.
Prof Lewis and his team singled out the "dragline" - the outer strand of the web - as the strongest of the six types of silk.
They spliced the DNA that creates the silk into a female goat's DNA, then waited for it to give birth and start lactating.
"(The splicing) turned out to be relatively easy as there are known gene promoters that only produce expression in the mammary gland during lactation," he said.
"Those were hooked up to our spider silk genes."
After the milk is collected, it's taken back to a laboratory where the silk protein is filtered out. It solidifies when exposed to air and is wound onto a roller.
Prof Lewis said the team collected about four metres of silk for every four drops of protein they gathered.
The pure material had a wide range of medicinal applications as sutures and binding agents - including ligament replacement - but its use could extend well beyond our hospitals.
"If it works, frankly one of the first applications is maybe fishing line," Prof Lewis said.
"I think we will be testing real world applications in less than two years (but) when they reach market is really beyond my control."
And in case you were wondering, no goats were harmed during the making of spider silk milk.
Prof Lewis said there was no evidence to suggest the goats in the experiment behaved any differently to regular goats, in either physiology or "psychology".
One day, the burden could be lifted even from goats.
Prof Lewis said the technology could have farm applications - he told Science Nation they were developing the same technology for alfalfa.
http://www.youtube.com/watch?v=ktgACq4zcAU&playnext=1&list=PL0A80C6A04C15983F
http://www.news.com.au/technology/sci-tech/meet-spider-goat-the-dna-enhanced-web-flinging-nanny-that-may-one-day-knit-your-bones/story-fn5fsgyc-1225867617374