Breakthrough ! ? Lasers stimulating tooth repair

[Bob]

Regrow instead of fillings ? Maybe it's on the horizon.

The research was led from Harvard's Wyss Institute and is being published today in Science Translational Medicine.

"Lasers are routinely used in medicine and dentistry, so the barriers to clinical translation are low," David Mooney, the research team's leader, says in a statement.

"It would be a substantial advance in the field if we can regenerate teeth rather than replace them."

Though the laser light was able to make stem cells turn into new dentin, it wasn't a direct change.

Instead, the laser set off a chain reaction, triggering one molecule, which triggered another, which finally set the stem cells in motion.

That the researchers have been able to track that reaction all the way back, they say, is critical, because it allows them to actually prove the infrared laser's efficacy, rather than adding further anecdotal evidence to the heap of literature on laser therapies for a clinical study.

The researchers believe that this method, known as low-level (Laser) light therapy, has the potential to trigger cells elsewhere in the body to similar reparative results.

The Experiment

Researchers say that your dentist might just point a laser at a damaged tooth, encouraging the tooth to regrow on its own.

It's been documented that Laser light causes reactions in the human body; some researchers have been trying to determine whether specific wavelengths of light might be able to trigger specific healing properties when focused on a certain area of the body.

In this case, the researchers pointed an infrared laser at a hole drilled into a rat's tooth and found that it encouraged dentin — the material that makes up a tooth's core — to grow back more than it otherwise would have.


ref: http://www.theverge.com/2014/5/28/5757952/tooth-regrowth-through-laser-therapy-is-possible-researchers-say


http://www.tampabay.com/resources/images/dti/rendered/2009/07/pb_laser0704_74706a_8col.jpg

(File photo, laser being used for Gum health)

[Bob]

TWO Laser wavelengths, in my experience were needed to do effective re-triggering of the stem cells..

A visible RED in the 635 nanometer wavelength area, and something in the 910 nanometer range.. alternating the modulation, stim/inhibit, stim/inhibit.. correct pulse timing, all that..

The combination creates new frequencies harmonics in the tissue, I wouldn't be surprised if the Golden mean / Fibonacci sequence is in there somewhere in the harmonics.. In other words, the harmonics trigger a "growth sequence"..

Here is an interesting PDF on the subject:


http://www.journal-imab-bg.org/statii-09/vol09_2_28-31str.pdf

[Carmody]

Underground bases, clone/super-soldier/hybrid manufacturing......part of the stated formula was.... light.

[Bob]

The two frequency lasers, one visible the other infrared are used.

Infrared laser stimulates, the visible red laser suppresses Dentin growth was the result of the tests.

My work on LASERS, was showing pulse sequences are needed. Specifically golden mean ratios of the harmonics of the lasers should be the method of regeneration.

From the PDF research notes:

"Especially strong stimulatory effect is determined by irradiation with monochromatic red and infrared light with 630-905 nm wavelength.

"The low level laser irradiation from red and near infrared light spectrum correspond exactly to relevant characteristic energy and absorbtion level in the respiratory chain. It acts directly on stimulating components of the so-called antenna pigments of the respiratory chain and manifest as an immediate effect cell vitalization by ATP mitochondrial production increase. "

The above explains how the cells are able to switch into increase energy production, using the ATP enzyme, where the laser light frequency is the exact trigger required to perform that action.


--------------------------------

from: http://stm.sciencemag.org/content/6/238/238ra69

Sci Transl Med 28 May 2014:
Vol. 6, Issue 238, p. 238ra69
Sci. Transl. Med. DOI: 10.1126/scitranslmed.3008234

The study was led by David Mooney at Harvard’s Wyss Institute:

"Non-ionizing, low-power laser (LPL) treatment can instead be used as a minimally invasive tool to activate an endogenous latent growth factor complex, transforming growth factor–β1 (TGF-β1), that subsequently differentiates host stem cells to promote tissue regeneration.

LPL (Low Power Laser) treatment induces a reactive oxygen species (ROS) in a dose-dependent manner, which, in turn, activates latent TGF-β1 (LTGF-β1) via a specific methionine residue (at position 253 on LAP). "

The significance of this shows STEM CELL differentiation is possible, or turning on a built-in repair mechanism which just required the proper energetic level and absorption wavelength.

The substance, TGF-β1, is a protein that controls proliferation, cellular differentiation, and other functions in most cells.

The nature of the TGF-β1 protein is that it must be TURNED OFF else uncontrolled growth can result in tumors, cancers, and a whole host of auto-immune diseases. The use of the RED LASER at 635- 638 nanometers appears to be capable of switching this protein back OFF.

[ThePythonicCow]

Lasers not ultrasound. :)
I moved the ultrasound posts to a new thread: Ultrasound stimulating tooth repair (http://projectavalon.net/forum4/showthread.php?71842-Ultrasound-stimulating-tooth-repair).

[Bob]

This PDF is an additional article backing up the use of LASERS in photodynamic therapy to evoke transforming growth factor–β1 (TGF-β1). This is needed to turn on Stem Cell differentiation, and then growing the proper cell, proper proteins to fulfill the creation of the desired form.


http://www.jpp.krakow.pl/journal/archive/06_13/pdf/387_06_13_article.pdf

Differentiated Stem Cells plus a viable appropriate "charged" scaffold, and the structure re-grows (or a new structure can be grown).

The key still is the appropriate other laser wavelength to then TURN OFF the stem cell activation, allowing now "normal" growth.

[Bob]

Since we are talking about stimulating STEM CELLs, it's probably a good idea to get more into, what is meant by "activation", and suppression. For me this is very black and white matter-of-fact, but possibly it's a bit deep in using the more technical terms as the scientists use in the reports mentioned above.

http://en.wikipedia.org/wiki/Tissue_engineering - this is a very simple explanation of Tissue Engineering methods as talked about in wiki..

"While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.).

"Often, the tissues involved require certain mechanical and structural properties for proper functioning.

"The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver).

"The term "regenerative medicine" is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues."

The techniques being talked about are capable of changing red blood cells into STEM CELLS, not requiring embryonic cells. :)

In each case, what is critical is to have a scaffold molecule or the "ideal shape" (the molecular signature "pattern") that determines the final SHAPE of the grown cellular structure.. Without the shaped scaffold, the tissue breaks into a massive tumor like shape (spheroid, or random, but not the desire shape of the final body part).

In a precision LASER induced differentiation, conversion back to the primary progenitor cells, the surrounding scaffold "pattern" is the rest of the tooth.

If one can imagine a bridge made out of LEGO blocks, taking out one block in the structure, and one has an idea of what type of block "pattern" is required to fill in a missing piece.

Turning on precise progenitor differentiation from let's say a red blood cell, to stem cell (progenitor) then in the presence in the electrical "holographic field" of the rest of the "tooth" (let's say in this case), the electronic pattern hold the electrical "scaffold" in which the needed steps to recreate a tooth can be created.

At some point, it is needed to precisely turn OFF the creation of progenitor cell formation, else one will develop tumors (random cell pattern clumps unable to be properly scaffolded into useful desired structure). The other laser wavelengths are used to do such things.

From my experience working with "holographic" electromagnetic pattern formation (loosely termed "flimsy" forcefields) I would believe a Laser in the range of 700 nanometers would be able to be sufficiently modulated with the desired pattern sequencing.

[vincent60]

Good morning Bob.Ijust received from Marina the message on my FB chat page. I don't know if what she ask to you it will be possible, but I understabd she's very interested in your posts and she doen't like to disturb me everytime she 'd like to contact you. So, here is her message for you. About me, I don't know if in the future we could have contacts. I like to say you that I love your country, and I visited USA 3 time ( N.Y. Orlando san Francisco L.A. ) and the last time in Tucson to visit a very nice fear of stones. You' re very kind with mw, see you one day, I hope. Here is Marina's message :
can you tell me your email address so that I can write and send email to you by personal computer me myself,without the help of my friend Vincent ,my email is (DELETED] and your? IT's only ot not to bother my friend all the time-thank you

MOD EDIT: Member's email address was deleted. Please share personal information via personal messages. --Marianne

[778 neighbour of some guy]

Vincent, you might want to copy your post to and in a PM to Bob and delete the post it self, you just copied a private address into a public forum, I don't know the dear lady, but it is not wise to exchange personal information like this.